Autumn stage:
As Michaelangelo, successfully complete The Dojo level.

Dojo Complete stage:
As Casey Jones, successfully complete the Turtle's Lair level.

Sewer Falls stage:
As Raphael, successfully complete the Sewer Falls level.

Sewers stage:
As Leonardo, successfully complete the Sewers level.

Shredder's Palace Exterior stage:
As Leonardo, successfully complete The Lab 12 level.

Subway stage:
As Leonardo, successfully complete the Sparring With Splinter level.

Waterfall stage:
As April, successfully complete the Turtle's Lair level.

April's Apartment stage:
As Donatello, successfully complete the April's Apartment 2 level.

April's adventure mode:
As Donatello, successfully complete the Ghetto level.

Casey Jones' adventure mode:
As Raphael, successfully complete the Subway level.

Foot Soldier's adventure mode:
As Raphael, successfully complete his adventure mode.

HUN's adventure mode:
As Michaelangelo, successfully complete his adventure mode.

Shredder's adventure mode:
As Donatello, successfully complete his adventure mode.

Splinter's adventure mode:
As Leonardo, successfully complete his adventure mode.

Casey Jones' jacket costume:
As April, successfully complete her adventure mode.

Casey Jones' red shorts costume:
As Casey Jones, successfully complete level the Ghetto level.

Shredder's gold costume:
As Shredder, successfully complete his adventure mode.