NOTE : Unlock the characters in the following order. Certain characters can only be unlocked after reaching a certain requirement.
NOTE 2 : It is recommandered that you play with each character on Hard difficulty setting so you do not need to backplay time after time
| Unlockable |
How to Unlock |
| Smoker |
Win the game with Roronoa Zoro (Hard mode suggested. Can be unlocked on any difficulty setting) |
| Wiper |
Win the game with Monkey.D.Luffy (Hard mode suggested. Can be unlocked on any difficulty setting) |
| Omu |
Win the game with Tony Tony Chopper (Hard mode suggested. Can be unlocked on any difficulty setting) |
| Mr 0 / Mr Crocodile |
Win the game with Nico. Robin (Hard mode suggested. Can be unlock on any difficulty setting) |
| Enel |
After unlocking all the other characters above win the game with Wiper and Monkey.D.Luffy on Hard difficulty setting. (Refer to unlocking Wiper) |
| Hina |
Once you've gained Enel, win the game with Usopp on Hard difficulty setting. |
| Mr 2 / Bon Clay |
After gaining Hina, win the game with Sanji on Hard difficulty setting. |
| Portgas.D.Ace |
After unlocking all characters above, beat Training mode with all of Luffy's crew and then win the game with Smoker on Hard difficulty setting. |
| Red Hair Shanks |
Once you've gained every other character, win the game on Hard mode using all of Luffy's crew then win the game once more with Luffy on Hard mode.. |
| Luffy's Crew |
For those who are wondering, Luffy's crew consists of Monkey.D.Luffy , Roronoa Zoro , Nami , Tony Tony Chopper , Usopp , Sanji and Nico. Robin |