| Unlockable |
How to Unlock |
| Normal Marine |
Defeat one in the Seaside Zone |
| Bazooka Marine |
Defeat one in the Seaside Zone |
| Marine Corps Chief |
Defeat one in the Jungle Zone |
| World Government Agent |
Defeat one in the Ruins Zone |
| Pistol Pirate |
Defeat one in the Jungle Zone |
| Sabre Pirate |
Defeat one in the Jungle Zone |
| Knuckle Pirate |
Defeat one in the Ruins Zone |
| Blue Demon General |
Defeat one in the Cave Zone |
| Cluster Demon Soldier |
Defeat one in the Cave Zone |
| Crimson Demon General |
Defeat one in the Cave Zone |
| Sword Demon Soldier |
Defeat one in the Cave Zone |
| Unlockable |
How to Unlock |
| Mihawk |
Beat him in story mode at the Fossil Beach area. |
| Arlong |
Beat him in story mode at the Cave area. |
| Eneru |
Beat him in story mode at the Ruins Zone area. |
| Smoker |
Beat him right before you enter Fossil Beach. |
| Spandam |
Beat Spandam at the Ruins zone he is the first boss. |
| Crocodile |
Beat him at the Cave area he is the second boss to reach him hit the wall with a character not a pick axe. |
| Kaku |
Defeat him in the Jungle area. |
| Portgas D. Ace |
Defeat him in the Mountain Zone |
| Shanks |
Defeat him in the Mountain Zone |
| Lucci |
Defeat him in the Dimension Zone |
| Aokiji |
Beat him in the Dimension Zone. |
| Rob Lucci |
Beat him in the Dimension Zone, he's the first boss. |
| Rampaging Chopper |
Beat him in Mountain Zone, you need a Revive Medicine to get to his arena. |
| Paulie |
Beat him at Cave Zone. |
| Vivi |
Beat her at Ruins Zone. |
| Calgara |
Beat him at Jungle Zone near a fishing spot. |
| Whitebeard |
Beat him in the Dimension Zone. Golden Whale is required to get to the arena. |
| Bon Clay |
Defeat him at the Seaside Zone area. |
